Vine Cane Compounds to Prevent Skin Cells Aging through Solid Lipid Nanoparticles
Adriana Pereira,
Maria João Ramalho,
Renata Silva,
Vera Silva,
Rita Marques-Oliveira,
Ana Catarina Silva,
Maria Carmo Pereira,
Joana A. Loureiro
Affiliations
Adriana Pereira
LEPABE (Laboratory for Process Engineering, Environment, Biotechnology and Energy), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
Maria João Ramalho
LEPABE (Laboratory for Process Engineering, Environment, Biotechnology and Energy), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
Renata Silva
Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
Vera Silva
Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
Rita Marques-Oliveira
Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
Ana Catarina Silva
Associate Laboratory i4HB—Institute for Health and Bioeconomy, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
Maria Carmo Pereira
LEPABE (Laboratory for Process Engineering, Environment, Biotechnology and Energy), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
Joana A. Loureiro
LEPABE (Laboratory for Process Engineering, Environment, Biotechnology and Energy), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
The long lifespan of the world’s population has been raising interest in the research for new solutions to delay the aging process. With the aim of skin aging prevention, solid lipid nanoparticles (SLNs) were developed in this work for the encapsulation of three lipophilic natural compounds extracted from vine cane—epigallocatechin gallate (EGCG), resveratrol and myricetin. The developed loaded-SLNs proved to be stable, maintaining their adequate physicochemical characteristics for 30 days. In addition, the loaded-SLNs formulations exhibited high encapsulation efficiencies and loading capacities and high intracellular antioxidant activity. The mixture of EGCG-loaded SLNs with resveratrol-loaded SLNs proved to have the highest protection against induced oxidative stress. The in vitro cytotoxicity of the loaded SLNs was also evaluated, showing that the developed formulations are biocompatible for concentrations up to 50 µg/mL and could be safe for use in cosmetics. The encapsulation of EGCG, resveratrol and myricetin in SLNs seems to be a suitable strategy for the delivery of these antioxidants to the skin, improving their bioavailability.
