Extraction and Identification of Chemical Compounds of Peganum harmala L. Seed Essential Oil by HS-SPME and GC-MS Methods

Abstract

Peganum harmala is a plant that belongs to the family Zygophyllaceae. In traditional medicine, this plant has therapeutic effects such as repelling intestinal worms, increasing sexual potency, increasing milk secretion, anti-rheumatism, regularizing, anti-parasitic, hypnotic, diaphoretic, anti-cancer and analgesic. The aim of this study was to identify the chemical composition of P. harmala seed essential oil. P. harmala seed essential oil was extracted by Head Space-SPME [HS-SPME] method and its chemical compounds were identified by GC-MS method. Totally, 41 chemical compounds were identified in P. harmala seed essential oil. According to the results, the most compounds of P. harmala L. seed essential oil includes 2,3-dimethyl benzofuran (28.32%), cis-linalool oxide (7.46%), [2E]- decenal (6.57%), 4aα,7β,7aα-nepetalactone (6.49%), 3-oxo-p-menth-1-en-7-al (6.36%) and trans- β- terpineol (5.86%), respectively.

Keywords

• Medicinal plant
• Essential oil
• Peganum harmala L.
• 3-dimethyl benzofuran
• Headspace Solid-Phase Microextraction (HS-SPME)
• Gas Chromatography Mass Spectrometry (GC-MS)