Comprehensive transcriptome analysis of AP2/ERFs in Osmanthus fragrans reveals the role of OfERF017-mediated organic acid metabolism pathway in flower senescence

Jing-Jing Zou; Jing-Jing Zou; Jing-Jing Zou; Jun Zhang; Xiaoqian Wang; Xiaoqian Wang; Xiaoqian Wang; Hui Xia; Hui Xia; Xiangling Zeng; Xiangling Zeng; Xiangling Zeng; Xuan Cai; Xuan Cai; Xuan Cai; Jie Yang; Jie Yang; Jie Yang; Jin Zeng; Jin Zeng; Zeqing Li; Zeqing Li; Zeqing Li; Guifu Zhang; Guifu Zhang; Hongguo Chen; Hongguo Chen; Hongguo Chen

doi:10.3389/fpls.2024.1467232

Frontiers in Plant Science (Sep 2024)

Comprehensive transcriptome analysis of AP2/ERFs in Osmanthus fragrans reveals the role of OfERF017-mediated organic acid metabolism pathway in flower senescence

Jing-Jing Zou,
Jing-Jing Zou,
Jing-Jing Zou,
Jun Zhang,
Xiaoqian Wang,
Xiaoqian Wang,
Xiaoqian Wang,
Hui Xia,
Hui Xia,
Xiangling Zeng,
Xiangling Zeng,
Xiangling Zeng,
Xuan Cai,
Xuan Cai,
Xuan Cai,
Jie Yang,
Jie Yang,
Jie Yang,
Jin Zeng,
Jin Zeng,
Zeqing Li,
Zeqing Li,
Zeqing Li,
Guifu Zhang,
Guifu Zhang,
Hongguo Chen,
Hongguo Chen,
Hongguo Chen

Affiliations

Jing-Jing Zou: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Jing-Jing Zou: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Jing-Jing Zou: Research Center for Osmanthus fragrans, Xianning Research Academy of Industrial Technology of Osmanthus fragrans, Xianning, China
Jun Zhang: Science Division, IGENEBOOK Biotechnology Co., Ltd., Wuhan, China
Xiaoqian Wang: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Xiaoqian Wang: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Xiaoqian Wang: College of Pharmacy, Hubei Universily of Science and Technology, Xianning, China
Hui Xia: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Hui Xia: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Xiangling Zeng: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Xiangling Zeng: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Xiangling Zeng: Research Center for Osmanthus fragrans, Xianning Research Academy of Industrial Technology of Osmanthus fragrans, Xianning, China
Xuan Cai: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Xuan Cai: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Xuan Cai: Research Center for Osmanthus fragrans, Xianning Research Academy of Industrial Technology of Osmanthus fragrans, Xianning, China
Jie Yang: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Jie Yang: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Jie Yang: Research Center for Osmanthus fragrans, Xianning Research Academy of Industrial Technology of Osmanthus fragrans, Xianning, China
Jin Zeng: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Jin Zeng: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Zeqing Li: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Zeqing Li: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Zeqing Li: Science Division, IGENEBOOK Biotechnology Co., Ltd., Wuhan, China
Guifu Zhang: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Guifu Zhang: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Hongguo Chen: National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China
Hongguo Chen: Osmanthus Innovation Center of National Engineering Research Center for Floriculture, Hubei University of Science and Technology, Xianning, China
Hongguo Chen: Research Center for Osmanthus fragrans, Xianning Research Academy of Industrial Technology of Osmanthus fragrans, Xianning, China

DOI: https://doi.org/10.3389/fpls.2024.1467232
Journal volume & issue: Vol. 15

Abstract

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Osmanthus fragrans is an ethylene-sensitive flower, and flower senescence was mediated by ethylene-responsive transcription factors (OfERFs). A total of 227 OfERFs were identified from O. fragrans, which were classified into five subfamilies: AP2 (35), DREB (57), ERF (125), RAV (6), and Soloist (4). Gene composition and structural analysis indicate that members of different subfamilies have different gene structures and conserved domains. Their gene promoter contains various functional responsive elements, including auxin, jasmonic acid, and other responsive elements. Among them, 124 OfAP2/ERF genes have expressed at any stage of flowering, and 10 of them may play roles in flowering or senescence. By comparative transcriptome analysis, OfAP2/ERFs affected by ethephon (ETH) and 5′-azacytidine (Aza) treatment were divided into three categories, which have various target gene sets. Importantly, these target gene sets participate in similar or different biological processes and metabolic pathways, suggesting that ethylene and DNA hypomethylation have crosstalk and a unique mechanism in regulating the flower senescence of O. fragrans. Co-expression analysis revealed that several key OfAP2/ERFs played a central role in organic acid metabolism and biosynthesis of branched-chain amino acids (BcAAs), among which OfERF017 was selected for further functional analysis. Overexpression of OfERF017 leads to significant enrichment of genes in organic acid metabolism pathways, which leads to a decrease in organic acid levels and promoting the flower senescence of O. fragrans. Together, these results give insights into the characteristics and functional analysis of OfAP2/ERF genes in O. fragrans.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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