Alpha-1-Acid Glycoprotein Quantification via Spatial Proximity Analyte Reagent Capture Luminescence Assay: Application as Diagnostic and Prognostic Marker in Serum and Effusions of Cats with Feline Infectious Peritonitis Undergoing GS-441524 Therapy
A. Katrin Helfer-Hungerbuehler,
Andrea M. Spiri,
Theres Meili,
Barbara Riond,
Daniela Krentz,
Katharina Zwicklbauer,
Katharina Buchta,
Anna-Maria Zuzzi-Krebitz,
Katrin Hartmann,
Regina Hofmann-Lehmann,
Marina L. Meli
Affiliations
A. Katrin Helfer-Hungerbuehler
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Andrea M. Spiri
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Theres Meili
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Barbara Riond
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Daniela Krentz
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany
Katharina Zwicklbauer
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany
Katharina Buchta
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany
Anna-Maria Zuzzi-Krebitz
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany
Katrin Hartmann
LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, LMU Munich, D-80539 Munich, Germany
Regina Hofmann-Lehmann
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Marina L. Meli
Clinical Laboratory, Department of Clinical Diagnostics and Services, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland
Until recently, the diagnosis of feline infectious peritonitis (FIP) in cats usually led to euthanasia, but recent research has revealed that antiviral drugs, including the nucleoside analog GS-441524, have the potential to effectively cure FIP. Alpha-1-acid glycoprotein (AGP) has been suggested as a diagnostic marker for FIP. However, AGP quantification methods are not easily accessible. This study aimed to establish a Spatial Proximity Analyte Reagent Capture Luminescence (SPARCLTM) assay on the VetBio-1 analyzer to determine the AGP concentrations in feline serum and effusion samples. Linearity was found in serial dilutions between 1:2000 and 1:32,000; the intra-run and inter-run precision was pKW pMWU TM assay offers a precise, fast and cost-effective method to measure the AGP concentrations in serum and effusion samples of feline patients. The monitoring of the AGP concentration throughout FIP treatment provides a valuable marker to evaluate the treatment’s effectiveness and identify potential relapses at an early stage.
