Overexpression of High-Mobility Motor Box 1 in the Blood and Tissues of Patients with Head and Neck Squamous Cell Carcinoma

Abstract

Introduction: Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world. Extra- and intra-cellular high-mobility motor box 1 (HMGB1) proteins are invovled in the pathogenesis and prognosis of cancer. Regarding this, the present study was conducted with the aim of investigating the expression of HMGB1 protein and mRNA levels in the blood, tumor tissue, and marginal normal tissue of patients with head and neck squamous cell carcinoma (HNSCC)usingthequantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). Materials and Methods: This study was performed on 88 patients with HNSCC, who referred to the otorhinolaryngology and oral pathology departments, affiliated to Mashhad University of Medical Sciences, Mashhad, Iran, and a group of healthy subjects (i.e., control group) matched in terms of age and gender.RNA was collected from fresh tumor tissues, marginal tissues, and blood, followed by the implementation of quantitative PCR on the specimens.Furthermore, the expression of HMGB1 in tumor and normal margins was evaluated by means of IHC. Thedata were analyzed in SPSS software. Results: According to the results,the expression levels of HMGB1 protein and mRNA were significantly higher in the tumor tissue than in the normal margin tissues (P

Keywords

• Biomarker
• Early diagnosis
• Head and neck squamous cell carcinoma (HNSCC)
• High-mobility motor box (HMGB1)
• Lymph node involvement