Nature Communications (Oct 2021)
Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
- Greta Bigelyte,
- Joshua K. Young,
- Tautvydas Karvelis,
- Karolina Budre,
- Rimante Zedaveinyte,
- Vesna Djukanovic,
- Elizabeth Van Ginkel,
- Sushmitha Paulraj,
- Stephen Gasior,
- Spencer Jones,
- Lanie Feigenbutz,
- Grace St. Clair,
- Pierluigi Barone,
- Jennifer Bohn,
- Ananta Acharya,
- Gina Zastrow-Hayes,
- Selgar Henkel-Heinecke,
- Arunas Silanskas,
- Ralf Seidel,
- Virginijus Siksnys
Affiliations
- Greta Bigelyte
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- Joshua K. Young
- Molecular Engineering, Corteva Agriscience™
- Tautvydas Karvelis
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- Karolina Budre
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- Rimante Zedaveinyte
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- Vesna Djukanovic
- Molecular Engineering, Corteva Agriscience™
- Elizabeth Van Ginkel
- Molecular Engineering, Corteva Agriscience™
- Sushmitha Paulraj
- Molecular Engineering, Corteva Agriscience™
- Stephen Gasior
- Molecular Engineering, Corteva Agriscience™
- Spencer Jones
- Molecular Engineering, Corteva Agriscience™
- Lanie Feigenbutz
- Molecular Engineering, Corteva Agriscience™
- Grace St. Clair
- Molecular Engineering, Corteva Agriscience™
- Pierluigi Barone
- Molecular Engineering, Corteva Agriscience™
- Jennifer Bohn
- Molecular Engineering, Corteva Agriscience™
- Ananta Acharya
- Molecular Engineering, Corteva Agriscience™
- Gina Zastrow-Hayes
- Molecular Engineering, Corteva Agriscience™
- Selgar Henkel-Heinecke
- Institute of Experimental Physics, Leipzig University
- Arunas Silanskas
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- Ralf Seidel
- Institute of Experimental Physics, Leipzig University
- Virginijus Siksnys
- Institute of Biotechnology, Life Sciences Center, Vilnius University
- DOI
- https://doi.org/10.1038/s41467-021-26469-4
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 8
Abstract
Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.
