Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages

Yifan Wang; Lamba Omar Sangaré; Tatiana C. Paredes-Santos; Musa A. Hassan; Shruthi Krishnamurthy; Anna M. Furuta; Benedikt M. Markus; Sebastian Lourido; Jeroen P. J. Saeij

doi:10.1038/s41467-020-18991-8

Nature Communications (Oct 2020)

Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages

Yifan Wang,
Lamba Omar Sangaré,
Tatiana C. Paredes-Santos,
Musa A. Hassan,
Shruthi Krishnamurthy,
Anna M. Furuta,
Benedikt M. Markus,
Sebastian Lourido,
Jeroen P. J. Saeij

Affiliations

Yifan Wang: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis
Lamba Omar Sangaré: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis
Tatiana C. Paredes-Santos: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis
Musa A. Hassan: College of Medicine and Veterinary Medicine, The University of Edinburgh
Shruthi Krishnamurthy: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis
Anna M. Furuta: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis
Benedikt M. Markus: Whitehead Institute for Biomedical Research
Sebastian Lourido: Whitehead Institute for Biomedical Research
Jeroen P. J. Saeij: Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis

DOI: https://doi.org/10.1038/s41467-020-18991-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 19

Abstract

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Using a genome-wide CRISPR screen, the authors here identify Toxoplasma genes that counteract the anti-parasitic activity of interferon gamma in murine macrophages. One of these genes, dense granule protein GRA45, is critical for the trafficking of GRA effectors and affects virulence in mice.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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