The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters

Zihan Wang; Xiaopeng Li; Jiejie Sun; Shuyi Mu; Wei Wu; Renle Chang; Lingling Wang; Linsheng Song

doi:10.1016/j.cirep.2025.200203

Comparative Immunology Reports (Jun 2025)

The involvement of CgCaspase-3 in regulating the expressions of proinflammatory cytokines through NF-κB/Rel signaling pathway in oysters

Zihan Wang,
Xiaopeng Li,
Jiejie Sun,
Shuyi Mu,
Wei Wu,
Renle Chang,
Lingling Wang,
Linsheng Song

Affiliations

Zihan Wang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China
Xiaopeng Li: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China
Jiejie Sun: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Corresponding authors at: Dalian Ocean University 52 Heishijiao Street, Dalian 116023, China.
Shuyi Mu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China
Wei Wu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China
Renle Chang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China
Lingling Wang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China
Linsheng Song: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Corresponding authors at: Dalian Ocean University 52 Heishijiao Street, Dalian 116023, China.

DOI: https://doi.org/10.1016/j.cirep.2025.200203
Journal volume & issue: Vol. 8
p. 200203

Abstract

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Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a prominent mediator of apoptosis and participates in the cell death signaling cascade. In our previous study, CgCaspase-3 identified from Crassostrea gigas was involved in CgGSDME-dependent pyroptosis. In the present study, CgCaspase-3 was demonstrated to regulate the expressions of proinflammatory cytokines through nuclear factor kappa B (NF-κB) signaling pathway. In haemocytes of Ac-DEVD-CHO-treated and CgCaspase-3-RNAi oysters, the nuclear translocation of NF-κB/Rel was inhibited at 24 h after Vibrio splendidus stimulation. The mRNA expressions of interleukins 17s (CgIL17–2, CgIL17–3, and CgIL17–6), tumor necrosis factor-1 (CgTNF-1), high mobility group box-1 protein (CgHMGB1) and interferon-like protein (CgIFNLP) were significantly decreased in haemocytes of CgCaspase-3-RNAi or CgRel-RNAi oysters, respectively. In addition, the phenomenon of gill swelling and cilia shedding was reduced in CgCaspase-3-RNAi oysters after V. splendidus stimulation. These results indicated that CgCaspase-3 was involved in regulating bacteria-induced inflammation through NF-κB/Rel signaling pathway.

Published in Comparative Immunology Reports

ISSN: 2950-3116 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology; Agriculture: Animal culture: Veterinary medicine
Website: https://www.sciencedirect.com/journal/comparative-immunology-reports

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