Optimization of culture conditions of caproic acid bacteria liquid for production and fermentation of artificial pit mud

Abstract

In order to obtain the bacterial liquid with high yield of caproic acid, the culture conditions of caproic acid bacterial liquid were optimized by single factor tests and Box-Behnken tests with caproic acid yield as evaluation index. The results showed that the optimal culture conditions of caproic acid bacterial liquid were as follows: anhydrous sodium acetate 8.0 g/L, yeast extract powder 1.5 g/L, ethanol addition 2%, initial pH 7, fermentation temperature 36 ℃, time 7 d. Under the optimized conditions, the yield of caproic acid could reached 4.9 g/L, which was 1.40 times higher than that before optimization.

Keywords

• artificial pit mud|caproic acid bacterial liquid|caproic acid yield|response surface method