Accelerated Oral Healing by <i>Angelica gigas</i> Nakai from Hot Melt Extrusion Technology: An In Vitro Study
Ju Ri Ye,
Ha Yeon Lee,
Yea-Jin Park,
Yong Kwon Chae,
Hyo-Jin An,
Jong-Suep Baek,
Ok Hyung Nam
Affiliations
Ju Ri Ye
Department of Pediatric Dentistry, Kyung Hee University Medical Center, College of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Ha Yeon Lee
Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Republic of Korea
Yea-Jin Park
Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University, Wonju 26339, Republic of Korea
Yong Kwon Chae
Department of Pediatric Dentistry, Kyung Hee University Medical Center, College of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Hyo-Jin An
Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea
Jong-Suep Baek
Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Republic of Korea
Ok Hyung Nam
Department of Pediatric Dentistry, Kyung Hee University Medical Center, College of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Background and Objectives: In spite of the oral environment being healing-prone, its dynamic changes may affect wound healing. The purpose of this study was to assess the oral wound healing effect of Angelica gigas Nakai (AG) prepared by hot-melt extrusion. Materials and Methods: Human gingival fibroblast (HGF) cells were treated with AG or AG via hot-melt extrusion (AGH) for 24 h to determine the optimal concentration. For evaluating the anti-inflammatory effect of AG and AGH, a nitric oxide assay was performed under lipopolysaccharide (LPS) stimulation. The wound-healing effects of AG and AGH were evaluated using cell proliferation/migration assays and wound-healing marker expression through qRT-PCR. Results: Both AG and AGH showed no cytotoxicity on HGH cells. Regarding nitric oxide production, AGH significantly decreased LPS-induced nitric oxide production (p p Conclusions: Within the limits of this study, AGH accelerated oral wound healing in vitro.
