eLife (May 2019)
Mechanism of completion of peptidyltransferase centre assembly in eukaryotes
- Vasileios Kargas,
- Pablo Castro-Hartmann,
- Norberto Escudero-Urquijo,
- Kyle Dent,
- Christine Hilcenko,
- Carolin Sailer,
- Gertrude Zisser,
- Maria J Marques-Carvalho,
- Simone Pellegrino,
- Leszek Wawiórka,
- Stefan MV Freund,
- Jane L Wagstaff,
- Antonina Andreeva,
- Alexandre Faille,
- Edwin Chen,
- Florian Stengel,
- Helmut Bergler,
- Alan John Warren
Affiliations
- Vasileios Kargas
- ORCiD
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Pablo Castro-Hartmann
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Norberto Escudero-Urquijo
- ORCiD
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Kyle Dent
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Christine Hilcenko
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Carolin Sailer
- ORCiD
- Department of Biology, University of Konstanz, Konstanz, Germany
- Gertrude Zisser
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
- Maria J Marques-Carvalho
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Simone Pellegrino
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Leszek Wawiórka
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom; Department of Molecular Biology, Maria Curie-Skłodowska University, Lublin, Poland
- Stefan MV Freund
- MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
- Jane L Wagstaff
- MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
- Antonina Andreeva
- MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
- Alexandre Faille
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- Edwin Chen
- ORCiD
- Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
- Florian Stengel
- ORCiD
- Department of Biology, University of Konstanz, Konstanz, Germany
- Helmut Bergler
- ORCiD
- Institute of Molecular Biosciences, University of Graz, Graz, Austria
- Alan John Warren
- ORCiD
- Cambridge Institute for Medical Research, Cambridge, United Kingdom; Department of Haematology, University of Cambridge, Cambridge, United Kingdom; Wellcome Trust–Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, United Kingdom
- DOI
- https://doi.org/10.7554/eLife.44904
- Journal volume & issue
-
Vol. 8
Abstract
During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase centre (PTC) completion that explains how integration of the last ribosomal proteins is coupled to release of the nuclear export adaptor Nmd3. Single-particle cryo-EM reveals that eL40 recruitment stabilises helix 89 to form the uL16 binding site. The loading of uL16 unhooks helix 38 from Nmd3 to adopt its mature conformation. In turn, partial retraction of the L1 stalk is coupled to a conformational switch in Nmd3 that allows the uL16 P-site loop to fully accommodate into the PTC where it competes with Nmd3 for an overlapping binding site (base A2971). Our data reveal how the central functional site of the ribosome is sculpted and suggest how the formation of translation-competent 60S subunits is disrupted in leukaemia-associated ribosomopathies.
Keywords