International Journal of General Medicine (Mar 2023)
The Value of Immunocytochemical Staining for the HPV E7 Protein in the Diagnosis of Cervical Lesions
Abstract
Mingjian Fei,1 Yawei Yu,1 Xiaolan Hu,1 Haimiao Xu,2 Fang Liu3 1Department of Pathology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of China; 2Department of Pathology, Zhejiang Provincial People’s Hospital, Hangzhou, Zhejiang, People’s Republic of China; 3Department of Gynecology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of ChinaCorrespondence: Mingjian Fei, Department of Pathology, The Second Hospital of Jiaxing, 1518 Huanchen North Road, Jiaxing, Zhejiang, 314000, People’s Republic of China, Email [email protected]: To investigate the value of immunocytochemical (ICC) staining for human papillomavirus (HPV) E7 protein (E7-ICC staining) as a new-generation immunological method in the cytological diagnosis of cervical lesions.Methods: The exfoliated cervical cell samples of 690 women were subjected to a liquid-based cytology test (LCT), high-risk HPV (HR-HPV) test, E7-ICC staining, and cervical biopsy for pathological diagnosis.Results: E7-ICC staining as a preliminary screening scheme for cervical precancerous lesions was comparable to the HR-HPV test in sensitivity and to the LCT in specificity. E7-ICC staining was advantageous in facilitating the secondary triage of HR-HPV-positive patients; therefore, this method can be used as an auxiliary scheme to routine LCT for diagnostic grading to improve the accuracy of cervical cytology.Conclusion: E7-ICC staining as a primary or auxiliary cytological screening scheme can effectively reduce the colposcopy referral rate.Keywords: cervical cancer, early screening for cervical cancer, cervical intraepithelial neoplasia, immunohistochemistry, HPV, E7 oncoprotein
