One health clones of multidrug-resistant Escherichia coli carried by synanthropic animals in Brazil
Elder Sano,
Fernanda Esposito,
Herrison Fontana,
Bruna Fuga,
Adriana Cardenas-Arias,
Quézia Moura,
Brenda Cardoso,
Gladyston C.V. Costa,
Tatiana C.M. Bosqueiro,
Juliana A. Sinhorini,
Eduardo de Masi,
Caroline C. Aires,
Nilton Lincopan
Affiliations
Elder Sano
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil; Corresponding authors at: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Fernanda Esposito
One Health Brazilian Resistance Project (OneBR), Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil
Herrison Fontana
One Health Brazilian Resistance Project (OneBR), Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil
Bruna Fuga
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil
Adriana Cardenas-Arias
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil
Quézia Moura
Federal Institute of Espírito Santo, Vila Velha, Brazil
Brenda Cardoso
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil
Gladyston C.V. Costa
Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil
Tatiana C.M. Bosqueiro
Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil
Juliana A. Sinhorini
Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil
Eduardo de Masi
Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil
Caroline C. Aires
Zoonoses Surveillance Division, Health Surveillance Coordination, Municipal Health Department, São Paulo, Brazil
Nilton Lincopan
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Corresponding authors at: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
WHO priority pathogens have disseminated beyond hospital settings and are now being detected in urban and wild animals worldwide. In this regard, synanthropic animals such as urban pigeons (Columba livia) and rodents (Rattus rattus, Rattus norvegicus and Mus musculus) are of interest to public health due to their role as reservoirs of pathogens that can cause severe diseases. These animals usually live in highly contaminated environments and have frequent interactions with humans, domestic animals, and food chain, becoming sentinels of anthropogenic activities. In this study, we report genomic data of Escherichia coli strains selected for ceftriaxone and ciprofloxacin resistance, isolated from pigeons and black rats. Genomic analysis revealed the occurrence of international clones belonging to ST10, ST155, ST224 and ST457, carrying a broad resistome to beta-lactams, aminoglycosides, trimethoprim/sulfamethoxazole, fluoroquinolones, tetracyclines and/or phenicols. SNP-based phylogenomic investigation confirmed clonal relatedness with high-risk lineages circulating at the human-animal-environmental interface globally. Our results confirm the dissemination of WHO priority CTX-M-positive E. coli in urban rodents and pigeons in Brazil, highlighting potential of these animals as infection sources and hotspot for dissemination of clinically relevant pathogens and their resistance genes, which is a critical issue within a One Health perspective.
