Nature Communications (Jun 2020)

Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion

  • Wenlong Ding,
  • Hongxia Zhao,
  • Yulin Chen,
  • Bin Zhang,
  • Yang Yang,
  • Jia Zang,
  • Jing Wu,
  • Shixian Lin

DOI
https://doi.org/10.1038/s41467-020-16898-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

Read online

Orthogonal aminoacyl-tRNA synthetase/tRNA pairs are crucial for the incorporation of unnatural amino acids in a site-specific manner. Here the authors use rational chimera design to create multiple efficient pairs that function in bacterial and mammalian systems for genetic code expansion.