Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

Mark Pogson; Cristina Parola; William J. Kelton; Paul Heuberger; Sai T. Reddy

doi:10.1038/ncomms12535

Nature Communications (Aug 2016)

Immunogenomic engineering of a plug-and-(dis)play hybridoma platform

Mark Pogson,
Cristina Parola,
William J. Kelton,
Paul Heuberger,
Sai T. Reddy

Affiliations

Mark Pogson: Department of Biosystems Science and Engineering, ETH Zurich
Cristina Parola: Department of Biosystems Science and Engineering, ETH Zurich
William J. Kelton: Department of Biosystems Science and Engineering, ETH Zurich
Paul Heuberger: Department of Biosystems Science and Engineering, ETH Zurich
Sai T. Reddy: Department of Biosystems Science and Engineering, ETH Zurich

DOI: https://doi.org/10.1038/ncomms12535
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

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Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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