International Journal of COPD (Sep 2019)

Association among genetic polymorphisms of GSTP1, HO-1, and SOD-3 and chronic obstructive pulmonary disease susceptibility

  • Du Y,
  • Zhang H,
  • Xu Y,
  • Ding Y,
  • Chen X,
  • Mei Z,
  • Ding H,
  • Jie Z

Journal volume & issue
Vol. Volume 14
pp. 2081 – 2088

Abstract

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Yong Du1,*, Hui Zhang2,*, Yan Xu3,*, Yi Ding1, Xuru Chen1, Zhoufang Mei1, Heyuan Ding4, Zhijun Jie1 1Department of Pulmonary and Critical Care Medicine, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai 200240, People’s Republic of China; 2Department of Endocrinology, People’s Hospital of Rizhao, Rizhao 276800, People’s Republic of China; 3Department of Laboratory Medicine, Rizhao Central Hospital, Rizhao 276800, People’s Republic of China; 4Department of Endocrinology, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai 200240, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhijun JieDepartment of Pulmonary and Critical Care Medicine, The Fifth People’s Hospital of Shanghai, Fudan University, No. 801 Heqing Road, Minhang District, Shanghai 200240, People’s Republic of ChinaTel +86 189 181 68602Fax +86 21 640 85875Email [email protected]: Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by incomplete reversible airflow limitation, which is associated with emphysema and chronic inflammation. Oxidative/antioxidant imbalance is one of the mechanisms of the current pathogenesis of COPD and several recent studies have attempted to uncover genetic causes of COPD and its progression. GST, HO-1, and SOD-3 are important susceptibility genes related to COPD.Methods: A total of 300 blood samples were included in two groups: Control group and COPD group. We genotyped 4 single nucleotide polymorphisms (SNPs) from these 3 genes in 150 COPD patients and 150 controls to analyze genetic polymorphisms and interactions with COPD-related quantitative traits using correlation analysis and multivariate logistic regression analysis.Results: The results indicated that genotype distributions and allele frequencies of GSTP1, HO-1, and SOD-3 were significantly different between the COPD and the control group, while there is no correlation between the polymorphism of GSTP1, HO-1, SOD3, and the different stages of COPD. Furthermore, multivariate logistic regression analysis indicated that COPD GSTP1-exon5 SNP and HO-1 (GT)n SNP are high-risk factors for COPD and there was interaction between GSTP1 exon5 SNPS and HO-1 (GT)n SNP. More important, the genotypes, AG, GG of GSTP1 exon5 and L/M*S, L/L of HO-1 (GT)n associated with increased 8-iso-prostaglandin F (2 alpha) (8-iso-PGF2) and malondialdehyde (MDA) concentration and decreased catalase (CAT) activity.Conclusion: Collectively, this study shows that genetic polymorphisms of GSTP1, HO-1, and SOD-3 are associated with COPD susceptibility.Keywords: chronic obstructive pulmonary disease, GSTP1, HO-1, SOD-3, SNPs

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