Biological Journal of Microorganism (Jan 2013)
Decolorization of Remazol Black-B by Halomonas sp. PTCC1417 isolated from Urmia lake: Optimization by Taguchi methodology
Abstract
Introduction: Azo dyes account as the most dyes of all textile dyestuffs produced, and are the most common dye in textile industries. Azo dyes-containing effluents from these industries have caused serious environmental pollution. Compared with chemical/physical methods, biological processes have received more interest.Materials and methods: In this study, we investigated effects of four factors including temperature, pH, dye concentration, salt concentration on decolorization of Remazol Black B by Halomonas sp. PTCC1714. The optimization of dye decolorization in 16 experiments with different conditions was statistically analyzed using Taguchi design in Qualitek-4 software.Results: The results showed that Halomonas sp. PTCC1714 was able to decolorize Remazol Black B in varying salt at 5–20% (w/v), pH at 5-9, dye concentration at 100-5000 ppm and temperature 31-40 ˚C. The optimum factor levels were a dye concentration of 100 ppm, salt concentation 10 % (w/v) and pH 9 and temperature 31˚C. The predicted value obtained for dye decolorization under these conditions was about 94%.Discussion and conclusion: It can be concluded that Halomonas sp. PTCC1714 has a high potential for decolorization of Remazol Black B from textile wastewater under different conditions.