Biosynthesis of Iron Nano-Particles by Bacillus Megaterium and Its Anti-Bacterial Properties

Abstract

BACKGROUND AND OBJECTIVE: According to the environmental pollution caused by chemical and physical methods of synthesis of nanoparticles and their incompatibility in medical, The aim of this study is biosynthesis of iron nano particles and its antibacterial activity. METHODS: In this experimental study, Bacillus megaterium PTCC1656 was cultured in nutrient broth medium, Then bacterial suspension was combined at a ratio of 1:1 with 0.1 M iron nitrate.Synthesis of iron nanoparticles confirmed with Uv-vis, XRD and SEM techniques. Antibacterial properties of nanoparticles were evaluated by disk diffusion method and dilution tube MIC (Minimum inhibitory concentration) on standard strains of Staphylococcus aureus PTCC 1431, Bacillus cereus 1015 PTCC and Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1571. FINDINGS: The peak of absorbtion for iron nanoparticle was 220 nm. Iron nanoparticles was cuboid shape. The average size of the nanoparticles was 40-60 nm. MIC was measured for Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa 0.031, 0.031, 0.0078, 0.015 mg/ml respectively. CONCLUSION: Results showed iron nanoparticle have antibacterial effect on pathogenic bacteria. The highest inhibition zone diameter by biological iron nitrate nanoparticles was observed for E. coli and the minimum diameter of inhibition was observed for Bacillus cereus

Keywords

• Bacillus Megaterium
• Anti-Bacterial Activity
• Iron Nanoparticles