Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, United States; Princeton Center for Theoretical Science, Princeton University, Princeton, United States
Julienne M LaChance
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, United States
Tom J Zajdel
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, United States
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, United States; Princeton Institute for the Science and Technology of Materials (PRISM), Princeton University, Princeton, United States
The coordination of cell proliferation and migration in growing tissues is crucial in development and regeneration but remains poorly understood. Here, we find that, while expanding with an edge speed independent of initial conditions, millimeter-scale epithelial monolayers exhibit internal patterns of proliferation and migration that depend not on the current but on the initial tissue size, indicating memory effects. Specifically, the core of large tissues becomes very dense, almost quiescent, and ceases cell-cycle progression. In contrast, initially-smaller tissues develop a local minimum of cell density and a tissue-spanning vortex. To explain vortex formation, we propose an active polar fluid model with a feedback between cell polarization and tissue flow. Taken together, our findings suggest that expanding epithelia decouple their internal and edge regions, which enables robust expansion dynamics despite the presence of size- and history-dependent patterns in the tissue interior.
