sFlt-1, Not PlGF, Is Related to Twin Gestation Choronicity in the First and Third Trimesters of Pregnancy
Szymon Kozłowski,
Anna Stelmaszczyk-Emmel,
Iwona Szymusik,
Aleksandra Saletra-Bielińska,
Robert Brawura-Biskupski-Samaha,
Paweł Pietruski,
Agnieszka Osińska,
Katarzyna Kosińska-Kaczyńska
Affiliations
Szymon Kozłowski
University Center for Woman and Newborn Health of the Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland
Anna Stelmaszczyk-Emmel
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Żwirki i Wigury 63A, 02-091 Warsaw, Poland
Iwona Szymusik
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland
Aleksandra Saletra-Bielińska
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland
Robert Brawura-Biskupski-Samaha
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland
Paweł Pietruski
Independent Public Complex of Healthcare Institutions in Pułtusk, 3 Maja 5, 06-100 Pułtusk, Poland
Agnieszka Osińska
2nd Department of Obstetrics and Gynecology, Center of Postgraduate Medical Education, Cegłowska 80, 01-809 Warsaw, Poland
Katarzyna Kosińska-Kaczyńska
2nd Department of Obstetrics and Gynecology, Center of Postgraduate Medical Education, Cegłowska 80, 01-809 Warsaw, Poland
Background: Preeclampsia occurs more often in dichorionic than in monochorionic twin pregnancy. We hypothesize that serum concentrations of biomarkers: placental growth factor (PlGF), serum soluble fms-like tyrosine kinase-1 (sFlt-1), and endoglin (Eng) differ between monochorionic and dichorionic twin pregnancies. Methods: A prospective observational study including 43 monochorionic and 36 dichorionic twin gestation was conducted. Blood samples were collected twice from all participants: between 11 + 0 and 13 + 6 and between 32 + 0 and 34 + 0 weeks of gestation. PlGF, sFlt-1 and Eng were measured using immnunoenzymatic assays. Results: We found a significantly higher concentration of sFlt-1 in dichorionic in comparison to monochorionic pregnancies in both the first and third trimesters. PlGF and sEng levels did not differ between mono- and dichorionic gestation in both study periods. sFlt-1 level was related to twin gestation chorionicity, while PlGF expression was not. PlGF, sFlt-1 and sEng concentrations increased significantly during gestation and were much higher in the third trimester compared to the values measured in the first trimester. Conclusions: Angiogenic biomarkers expression differ between dichorionic and monochorionic twin pregnancy. The sFlt-1 level is related to chorionicity of a twin gestation.
