Nature Communications (Mar 2018)

Rapid measurement of inhibitor binding kinetics by isothermal titration calorimetry

  • Justin M. Di Trani,
  • Stephane De Cesco,
  • Rebecca O’Leary,
  • Jessica Plescia,
  • Claudia Jorge do Nascimento,
  • Nicolas Moitessier,
  • Anthony K. Mittermaier

DOI
https://doi.org/10.1038/s41467-018-03263-3
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 7

Abstract

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There is growing evidence that the kinetics of interactions between inhibitors and their targets can strongly impact therapeutic efficacy. Here the authors describe an isothermal titration calorimetry-based method that can rapidly quantify inhibition kinetics and measure sub-nM binding affinities.