The immunological effects of the polyamine oxidase enzyme partially cleared from the cerebrospinal fluid against secondary secondary cystitis in white mice. Coagulation factor and delayed hypersensitivity

Abstract

The study included immune response to infection with secondary hydatid disease in BALB/c mice activated by partially purified cerebrospinal fluid-polyamine oxidase (CSF-PAO) with spermine and infected with protoscoleces of Echinococcus granulosus. The pathological changes occurred in mice activated by different concentrations of PA0(200-1600 )lg/1 Ogm body weight) with constant concentration (200)-lg/1 Ogm body weight) of substrate spermine, and by the same concentration of spermine alone, were followed in comparison with positive control group along one month, depending on certain criteria included changes in the non-specific and specific immuneresponse represented by phagocytosis and delayed-type hypersensitivity, respectively. The results revealed an increase in the non-specific (innate) and specific (cellular) immune response, represented by an increase in the rate of phagocytic index and foot pad thickness, respectively, in activated mice with PAO-Spm system, in comparison with +ve control group.Whereas an increase in the non-specific (innate), but no change in specific (cellular) immune responses occurred with mice activated with Spm alone.It may , therefore, be concluded here that P AO isolated from CSF with Spm could be considered as an effective immunumodulator against infection with ·secondary hydatid disease.

Keywords

• immunological
• polyamine oxidase
• cerebrospinal