Suitability of the date of grapevine tissue sampling for tests of GFLV and ArMV presence using ELISA method

Abstract

Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) are two important agents from the Nepoviruses genus. At present, serodiagnostic method is one of routine methods of virus pathogens testing also in grapevines. The aim of this study was to determine a suitable date of grapevine tissue sampling using the ELISA method and to follow visible symptoms of GFVL and ArMV infestation of vines. Positive results of tests for the occurrence of GFLV were obtained in the following growth stages: B (intensive growth – growth apex), C (beginning of flowering – young leaves) and D (beginning of flowering – inflorescence). Samples evaluated by ELISA method as positive for ArMV were found out in growth stages B (intensive growth – growth apex) and C (beginning of flowering – inflorescence). In all other stages the results of performed tests were negative. It is concluded that the stage B (intensive growth – growth apex) seems to be the most suitable for sampling of grapevine tissue.

Keywords

• grapevine
• grapevine virus disease
• GFLV
• ArMV
• ELISA method
• visual symptoms