EFFICIENCY OF DIFFERENT TECHNIQUES FOR ANTIBODY DETERMINATION AND TESTING OF ANTIGEN-BINDING LYMPHOCYTES IN DIAGNOSTICS OF BRUCELLOSIS

Abstract

Abstract. The aim of this study was to compare the efficiency of different methods applied for the diagnostics of brucellosis. Huddlson and Wright reactions, RoseBengal test, ELISA-based test system (IgG determination), and a test for antigen-binding lymphocytes (ABL) were used. There was shown that the specificity of tests was identical for either reactions based on detection of antibodies with commercial immune reagents and ELISA IgG. The sensitivity of ABL test is, however, superior to all the reactions based on antibody determination. The ABL test has advantages when compared with antibody detection techniques. This method permits early monitoring of brucellosis treatment/its efficiency since early terms, as well as differentiation between positive and false positive results of antibody determination in pregnant womеn.

Keywords

• brucellosis
• diagnostics
• antibody
• antigen-binding lymphocytes