Fungal Infected Adipose Stem Cells: The Effects of Novel Lipo-Niosome Nanoparticles Loaded with Amphotericin B and Thymus Essential Oil

Fardin Rahimi; Ghasem Amoabediny; Hossein Sabahi; Behrouz Zandieh-Doulabi

doi:10.22074/cellj.2022.7967

Cell Journal (Jul 2022)

Fungal Infected Adipose Stem Cells: The Effects of Novel Lipo-Niosome Nanoparticles Loaded with Amphotericin B and Thymus Essential Oil

Fardin Rahimi,
Ghasem Amoabediny,
Hossein Sabahi,
Behrouz Zandieh-Doulabi

Affiliations

Fardin Rahimi: Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Ghasem Amoabediny: Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran
Hossein Sabahi: Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Behrouz Zandieh-Doulabi: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), The University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Amsterdam, The Netherlands

DOI: https://doi.org/10.22074/cellj.2022.7967
Journal volume & issue: Vol. 24, no. 7
pp. 391 – 402

Abstract

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Objective: In this study, we aimed to develop new Lipo-niosomes based nanoparticles loaded with Amphotericin B(AmB) and Thymus Essential Oil (TEO) and test their effectiveness in the treatment of fungal-infected human adipose stem cells (hASCs).Materials and Methods: In this experimental study, optimal formulation of AmB and TEO loaded lipo-niosome (based on lipid-surfactant thin-film hydration method) was chemically, and biologically characterized. Therefore, encapsulation capacity, drug release, size, and the survival rate of cells with different concentrations of free and encapsulated AmB/ TEO were evaluated using the MTT method, and its antifungal activity was compared with conventional AmB.Results: Lipo-Niosome containing Tween 60 surfactant: cholesterol: Dipalmitoyl phosphatidylcholine (DPPC): Polyethylene glycol (PEG) with a ratio of 20:40:60:3 were chosen as optimal formulation. Lipo-Niosomes entrapment efficiency was %94.15. The drug release rate after 24 hours was %52, %54, and %48 for Lipo-AmB, Lipo-TEO, and Lipo-AmB/TEO, respectively. Physical and chemical characteristics of the Lipo-Niosomes particles indicated size of 200 nm and a dispersion index of 0.32 with a Zeta potential of -24.56 mv. Furthermore, no chemical interaction between drugs and nano-carriers was observed. The cell viability of adipose mesenchymal stem cells exposed to 50 μg/ml of free AmB, free TEO, and free AmB/TEO was %13.4, %58, and %36.9, respectively. Whereas the toxicity of the encapsulated formulas of these drugs was %48.9, %70.8, and %58.3 respectively. The toxicity of nanoparticles was very low (%8.5) at this concentration. Fluorescence microscopic images showed that the antifungal activity of Lipo- AmB/TEO was significantly higher than free formulas of AmB, TEO, and AmB/TEO.Conclusion: In this study, we investigated the efficacy of the TEO/AmB combination, in both free and encapsulatedniosomal form, on the growth of fungal infected-hASCs. The results showed that the AmB/TEO-loaded Lipo-Niosomes can be suggested as a new efficient anti-fungal nano-system for patients treated with hASCs.

Published in Cell Journal

ISSN: 2228-5806 (Print); 2228-5814 (Online)
Publisher: Royan Institute (ACECR), Tehran
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine; Science
Website: http://celljournal.org/

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