Development, Physicochemical Characterization and In Vitro Anti-Inflammatory Activity of Solid Dispersions of α,β Amyrin Isolated from Protium Oilresin
Walter Ferreira da Silva Júnior,
Jonas Gabriel de Oliveira Pinheiro,
Danielle Lima Bezerra de Menezes,
Natan Emanuell de Sobral e Silva,
Patrícia Danielle Oliveira de Almeida,
Emerson Silva Lima,
Valdir Florêncio da Veiga Júnior,
Eduardo Pereira de Azevedo,
Ádley Antonini Neves de Lima
Affiliations
Walter Ferreira da Silva Júnior
Pharmacy Department, Health Sciences Center, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59012-570, Rio Grande do Norte (RN), Brazil
Jonas Gabriel de Oliveira Pinheiro
Pharmacy Department, Health Sciences Center, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59012-570, Rio Grande do Norte (RN), Brazil
Danielle Lima Bezerra de Menezes
Pharmacy Department, Health Sciences Center, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59012-570, Rio Grande do Norte (RN), Brazil
Natan Emanuell de Sobral e Silva
Pharmacy Department, Health Sciences Center, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59012-570, Rio Grande do Norte (RN), Brazil
Patrícia Danielle Oliveira de Almeida
Department of Pharmacy, Laboratory of Biological Activity, Federal University of Amazonas, Manaus 69077-000, AM, Brazil
Emerson Silva Lima
Department of Pharmacy, Laboratory of Biological Activity, Federal University of Amazonas, Manaus 69077-000, AM, Brazil
Valdir Florêncio da Veiga Júnior
Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Manaus 69077-000, AM, Brazil
Eduardo Pereira de Azevedo
Graduate Program in Biotechnology, Laureate International Universities—UnP, Natal 59056-000, RN, Brazil
Ádley Antonini Neves de Lima
Pharmacy Department, Health Sciences Center, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59012-570, Rio Grande do Norte (RN), Brazil
α,β Amyrin (ABAM) is a natural mixture of pentacyclic triterpenes that has shown a variety of pharmacological properties, including anti-inflammatory effect. ABAM is isolated from Burseraceae oilresins, especially from the Protium species, which is commonly found in the Brazilian Amazon. This work aimed to develop solid dispersions (SD) of ABAM with the following hydrophilic polymers: polyvinylpyrrolidone (PVP-K30), polyethylene glycol (PEG-6000) and hydroxypropylmethylcellulose (HPMC). The SDs were prepared by physical mixture (PM), kneading (KND) and rotary evaporation (RE) methods. In order to verify any interaction between ABAM and the hydrophilic polymers, physicochemical characterization was performed by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), powder X-ray diffraction (XRD), thermogravimetry (TG) and differential scanning calorimetry (DSC) analysis. Furthermore, an in vitro anti-inflammatory assay was performed with ABAM alone and as SDs with the hydrophilic polymers. The results from the characterization analysis show that the SDs were able to induce changes in the physicochemical properties of ABAM, which suggests interaction with the polymer matrix. In vitro anti-inflammatory assay showed that the SDs improved the anti-inflammatory activity of ABAM and showed no cytotoxicity. In conclusion, this study showed the potential use of SDs as an efficient tool for improving the stability and anti-inflammatory activity of ABAM without cytotoxicity.
