Data on clinical significance of GAS2 in colorectal cancer cells
Chun-Chao Chang,
Chi-Cheng Huang,
Shung-Haur Yang,
Chih-Cheng Chien,
Chia-Long Lee,
Chi-Jung Huang
Affiliations
Chun-Chao Chang
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan; Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
Chi-Cheng Huang
School of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Breast Center, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Taipei Medical University, Taipei, Taiwan
Shung-Haur Yang
Department of Surgery, Taipei-Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang Ming University, Taipei, Taiwan
Chih-Cheng Chien
School of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Department of Anesthesiology, Cathay General Hospital, Taipei, Taiwan
Chia-Long Lee
School of Medicine, Fu Jen Catholic University, New Taipei, Taiwan; Department of Internal Medicine, Cathay General Hospital, Taipei, Taiwan
Chi-Jung Huang
Department of Medical Research, Cathay General Hospital, Taipei, Taiwan; Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan; Corresponding author at: Department of Medical Research, Cathay General Hospital, No. 32, Lane 160, Jiancheng Road, Sijhih District, New Taipei 22174, Taiwan, ROC.
The growth arrest-specific 2 (GAS2) was cloned and found to be upregulated in the feces of recurrent CRC patients. This overexpressed GAS2 induced different patterns of gene expressions in CRC cells. Briefly, one cell proliferation marker, Ki-67 antigen (Ki-67), was upregulated in the cells with overexpressed GAS2, “Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer” [1]. Whereas, the expression of another cell proliferation marker, proliferating cell nuclear antigen (PCNA), changed insignificantly [1]. In addition, the mRNA level of one cyclin involving in both cell cycle G1/S and G2/M transitions was also not affected by GAS2 overexpression, “Cdc20 and Cks direct the spindle checkpoint-independent destruction of cyclin A” [2]. The experimental design and procedures in this article can be helpful for understanding the molecular significance of GAS2 in SW480 and SW620 CRC cells.
