A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice

Xiaodong Tian; Yiyu Zhang; Xinyu Li; Ying Xiong; Tianchen Wu; Hui-Wang Ai

doi:10.1038/s41467-022-31673-x

Nature Communications (Jul 2022)

A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice

Xiaodong Tian,
Yiyu Zhang,
Xinyu Li,
Ying Xiong,
Tianchen Wu,
Hui-Wang Ai

Affiliations

Xiaodong Tian: Department of Molecular Physiology and Biological Physics, University of Virginia
Yiyu Zhang: Department of Molecular Physiology and Biological Physics, University of Virginia
Xinyu Li: Department of Molecular Physiology and Biological Physics, University of Virginia
Ying Xiong: Department of Molecular Physiology and Biological Physics, University of Virginia
Tianchen Wu: Department of Molecular Physiology and Biological Physics, University of Virginia
Hui-Wang Ai: Department of Molecular Physiology and Biological Physics, University of Virginia

DOI: https://doi.org/10.1038/s41467-022-31673-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Bioluminescence imaging of neuronal activity is desired. Here the authors report a luciferase prosubstrate activatable in vivo by nonspecific esterase and engineer a bioluminescent indicator with responsiveness to calcium ions: integration of these components enabled imaging of neuronal activity in mice.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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