Cell Journal (Jan 2008)
Evaluation of Bax Encoding Plasmid For Increasing Efficacy of DNA Vaccine Plasmid Encoding gB of Herpes Simplex Virus Type 1
Abstract
Objective: Evaluation of Bax encoding plasmid for increasing efficacy of DNAvaccine plasmid encoding gB of Herpes Simplex Virus type 1.Materials and Methods: We compared three different dosages of Bax encodingplasmid (pcbax) including 10, 25 and 50 μg of plasmid DNA. They were co-injectedineradermally with glycoprotein B (gB) of herpes simplex virus (HSV)-1 encodedplasmid (pcgB) in C57BL/6 mice to elicit immune responses to protect against lethalHSV-1 challenge. Immune responses to the antigen were assessed by lymphocyteproliferative responses and cytokine (INF-γ and IL-4) release assays.Results: The study demonstrates that the mice immunized with 25 μg pcbaxtogether with pcgB have more efficient protection than the mice immunized with 10and 50 μg of pcbax and pcgB. Analysing of cell-mediated responses show that themice immunized with 25μg pcbax and pcgB induce stronger lymphocyte proliferativeresponses and higher levels of INF-γ and IL-4 compared to the mice are received 10and 50 μg of pcbax and pcgB.Conclusion: The data show that co-immunization with 25 μg of pcbax and pcgBincrease immune responses compared to 10 and 50 μg of pcbax and pcgB. Thiscan be considered a promising approach for development an efficient DNA vaccineagainst HSV-1 or other pathogens.
