Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells

Gualtiero Alvisi; Marta Trevisan; Giulia Masi; Vanessa Canel; Luciana Caenazzo; Patrizia Nespeca; Luisa Barzon; Enzo Di Iorio; Vanessa Barbaro; Giorgio Palù

Stem Cell Research (Apr 2018)

Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells

Gualtiero Alvisi,
Marta Trevisan,
Giulia Masi,
Vanessa Canel,
Luciana Caenazzo,
Patrizia Nespeca,
Luisa Barzon,
Enzo Di Iorio,
Vanessa Barbaro,
Giorgio Palù

Affiliations

Gualtiero Alvisi: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Marta Trevisan: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy; Corresponding author.
Giulia Masi: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Vanessa Canel: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Luciana Caenazzo: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Patrizia Nespeca: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Luisa Barzon: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Enzo Di Iorio: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy
Vanessa Barbaro: Fondazione Banca degli Occhi del Veneto, 30174 Venice, Italy
Giorgio Palù: Department of Molecular Medicine, University of Padova, 35121 Padua, Italy

Journal volume & issue: Vol. 28
pp. 177 – 180

Abstract

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Human oral mucosa epithelial stem cells (hOMESCs) were obtained from a fresh oral biopsy collected from a healthy subject at the Fondazione Banca degli Occhi del Veneto (FBOV). An integration-free reprogramming protocol was applied exploiting episomal plasmids transfected into cells using a Nucleofector device. Around day 20 post transfection, several human induced pluripotent stem cell (hiPSC) colonies were manually picked and expanded. One of these (UNIPDi001-A-hiPSCs) expressed undifferentiated state marker alkaline phosphatase along with a panel of pluripotency state markers and was able to differentiate into the derivatives of all the three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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