Inhibition of allicin in Eca109 and EC9706 cells via G2/M phase arrest and mitochondrial apoptosis pathway

Fenrong Chen; Hong Li; Yan Wang; Meili Gao; Yan Cheng; Dong Liu; Miao Jia; Jun Zhang

Journal of Functional Foods (Aug 2016)

Inhibition of allicin in Eca109 and EC9706 cells via G2/M phase arrest and mitochondrial apoptosis pathway

Fenrong Chen,
Hong Li,
Yan Wang,
Meili Gao,
Yan Cheng,
Dong Liu,
Miao Jia,
Jun Zhang

Affiliations

Fenrong Chen: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Hong Li: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Yan Wang: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Meili Gao: Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China
Yan Cheng: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Dong Liu: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Miao Jia: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China
Jun Zhang: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China; Corresponding author. Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, The Key Laboratory of Gastrointestinal Motility Disorders of Shaanxi Province, Xi'an, Shaanxi Province 710004, China. Fax: +86 29 87679660.

Journal volume & issue: Vol. 25
pp. 523 – 536

Abstract

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The effects and underlying molecular mechanisms of allicin on oesophageal squamous cell carcinoma (ESCC) are unclear. Herein, we investigated the effect and action mechanism of allicin in the ESCC cells of Eca-109 and EC9706. The cell viability and invasion ability of the two ESCC cells in allicin group were significantly decreased after allicin treatment. Allicin reduced the percentage of G0/G1 and S phase and increased the population of G2/M phase in a dose-dependent manner. The expression levels were significantly increased for p53, p21, CHK1, and decreased for cyclinB after allicin treatment. The apoptotic rate was significantly increased and accompanied by Cyt c release from the mitochondria to the cytosol, as well as Bax, caspase-3 and cleaved caspase-9 which were significantly increased. Furthermore, suppression of tumour growth was evident in vivo. These results suggest that allicin can serve as a novel and effective anti-oesophageal cancer agent for the treatment of ESCC.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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