PHYSICO-CHEMICAL VERIFICATION OF THE APPLICATION OF THE CYTOSTATIC IMMOBILAZED FORM IN OVARIAN CANCER THERAPY

Abstract

The aim of the research is to study the interaction between “Linteks-Mesogel” and paclitaxel, antitumor pharmaceutical substances. The object of the research is “Linteks-Mesogel”, a concentrated water solution of sodium salt of carboxymethyl cellulose (Na-CMC). It can be used as an inert polymer carrier for some pharmaceutical substances and has an anti-spasmodic effect. FTIR and UV-spectroscopy methods were used to investigate the interaction. The UV-spectroscopy showed that light absorbance maxima for paclitaxel and its mixture with “Linteks-Mesogel” water solution are identical. The FTIR data confi rm absence of covalent bonding between the molecules of sodium salt of carboxymethyl cellulose and the molecules of paclitaxel. A shift to 1642 cm–1 can only be observed in case of the absorption band at 1636 cm–1, which is attributed to stretching vibrations of the carbonyl groups of sodium salt of carboxymethyl cellulose in associated water solutions. It proves that there is destabilization of the association of Na-CMC macromolecules in aqua solutions with paclitaxel molecules which causes the formation of self-organizing systems. The transmission electron microscopy (TEM) showed that the associates of Na-CMC with paclitaxel have a complex composition. The associates consisting of a number of Na-CMC macromolecules and paclitaxel molecules form self-organizing systems with a strictly defi ned architecture. To sum up, the obtained data allow us to conclude that “Linteks-Mesogel” can be used as polymer carrier for paclitaxel in the ovarian cancer therapy providing a prolonged action and a decreased toxicity of paclitaxel.

Keywords

• «Linteks-Mesogel»
• paclitaxel
• UV-spectroscopy
• FTIR
• transmisson electron microscopy