Медицинский совет (Aug 2021)

Modern immunopharmacological methods of discovering new properties of benzydamine by its influence on innate and adaptive immune cells

  • A. N. Kazimirskii,
  • J. M. Salmasi,
  • G. V. Poryadin,
  • I. V. Kukes

DOI
https://doi.org/10.21518/2079-701X-2021-11-111-117
Journal volume & issue
Vol. 0, no. 11
pp. 111 – 117

Abstract

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Abstract Introduction. Considering the development of technologies and techniques, it is highly relevant today to continue studying the features of interaction of drugs developed and introduced into clinical practice in the 1960s and 1980s with the elements of the immune system located in the mucosa, since many of these drugs have local dosage forms. One such drug is benzidamine hydrochloride, registered as a nonsteroidal anti-inflammatory drug with extended pharmacodynamic properties.Objective. To evaluate the effect of benzidamine hydrochloride preparation on the cells of innate and acquired immunity located in the mucosa in an in vitro model.Materials and Methods. Cell fractions of neutrophils isolated from patients with infectious inflammation were used in the study. Sterile isolated neutrophils from the venous blood of patients were transferred into RPMI-1640 medium, where the investigated pharmacological drug was added, and incubation with cells was performed. Fluorescence microscopy was used to detect and count neutrophil extracellular traps. Cell fractions of lymphocytes isolated from patients with infectious inflammation were also used in the study. A commercial drug containing benzidamine hydrochloride, Tantum Verde (Angelini Pharma S.p.a., Italy), was used in the study.Results and discussion. Benzidamine causes significant inhibition of neutrophil extracellular trap formation. This suggests that at the beginning of application the drug enhances the antimicrobial response of the body, and then as the concentration decreases it demonstrates classic anti-inflammatory properties. The drug also enhances the neutrophil extracellular trap structure. The total number of B-lymphocytes tends to normalize under the effect of benzidamine. These data allow us to conclude about the ability of the drug to attenuate the severity of the inflammatory process, apparently by inhibiting the action of inflammatory cytokines.Conclusion. In the course of the in vitro study it was shown that benzidamine hydrochloride (Tantum Verde) has immunomodulatory properties against innate immunity cells – neutrophils, and its anti-inflammatory properties against adaptive immunity cells – lymphocytes – were confirmed.

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