Nature Communications (Jul 2018)

Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance

  • Jone Michelena,
  • Aleksandra Lezaja,
  • Federico Teloni,
  • Thomas Schmid,
  • Ralph Imhof,
  • Matthias Altmeyer

DOI
https://doi.org/10.1038/s41467-018-05031-9
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 16

Abstract

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Methods to study anti-cancer drugs cytotoxicity are often low throughput and rely on population average. Here the authors present an automated image-based cytometry method to quantify multiple cytotoxicity parameters in single cells, and use it to study the effect of PARP inhibitors in cancer cells.