Polymorphism of tumor suppressor gene (p53) Codon 72 in Iraqi patients with acute myocardial infarction

Abstract

Objectives According to previous studies, the aim of the presented work is to investigate the possible association between the codon 72 polymorphism (Pro72Arg, rs1042522) of the tumor suppressor gene (p53) with acute myocardial infarction (AMI) in Iraqi patients of Karbala province. Methods A total of 58 smokers and non-smokers patients with AMI diagnosed by clinical history, electrocardiography and troponin I level and another 35 healthy controls were included in this study. The (Pro72Arg) polymorphism of the p53 gene was evaluated by molecular techniques. Results The genotype distribution for the Pro72Arg variant of the p53 gene in AMI non-smokers patients (PP: n = 9, 47.4%; RP: n = 2, 10.5%; RR: n = 8, 42.1%) and controls (PP: n = 9, 50.0%; RP: n = 9, 50.0%; RR: n = 0, 0.0%) was significantly different (p = 0.002). and the genotype distribution for the Pro72Arg variant of the p53 gene in AMI smokers patients (PP: n = 20, 51.3%; RP: n = 1, 2.6%; RR: n = 18, 46.2%) and controls (PP: n = 14, 82.4%; RP: n = 2, 11.8%; RR: n = 1, 5.9%) was significantly different (p = 0.009). Conclusion These findings suggest that the Pro72Arg polymorphism of tumor suppressor p53 gene is associated with AMI patient studies.

Keywords

• AMI, PCR, RFLP, polymorphism