Salinity gradients drove the gut and stomach microbial assemblages of mud crabs (Scylla paramamosain) in marine environments

Hafiz Sohaib Ahmed Saqib; Ye Yuan; Syed Shabi Ul Hassan Kazmi; Shengkang Li; Huaiping Zheng; Yueling Zhang; Mhd Ikhwanuddin; Hongyu Ma

Ecological Indicators (Jul 2023)

Salinity gradients drove the gut and stomach microbial assemblages of mud crabs (Scylla paramamosain) in marine environments

Hafiz Sohaib Ahmed Saqib,
Ye Yuan,
Syed Shabi Ul Hassan Kazmi,
Shengkang Li,
Huaiping Zheng,
Yueling Zhang,
Mhd Ikhwanuddin,
Hongyu Ma

Affiliations

Hafiz Sohaib Ahmed Saqib: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Ye Yuan: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Syed Shabi Ul Hassan Kazmi: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Shengkang Li: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Huaiping Zheng: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Yueling Zhang: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
Mhd Ikhwanuddin: STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China; Higher Institute Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus 21030, Terengganu, Malaysia
Hongyu Ma: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China; Corresponding author at: Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.

Journal volume & issue: Vol. 151
p. 110315

Abstract

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Salinity changes have profound effects on the development, physiology, and molecular activity, as well as the associated microbiome of organisms living in marine and estuarine ecosystems. The associated microbiomes of aquatic organisms are reported to support key physiological functions such as nutrition, reproduction, behavior, and health. The mud crabs, a euryhaline crustacean species, has extra-ordinary ability to survive in a wide range of salinities. Although mud crabs are exposed to diverse salinity conditions, little is known about how these variations affect the symbiotic bacteria they harbor in each environment. This study aimed to deciphering the associated microbiome assembly of mud crabs as well elucidating the potential implications of different salinity conditions in this process. Here, we used high-throughput sequencing to examine how the assemblages of bacteria in the gut of mud crabs are shaped by different salinity gradients. A total of 57 bacterial phyla were detected with Firmicutes and Proteobacteria dominant. A greater difference of microbiome in the gut than stomach of mud crabs indicating guts were more sensitive to different salinity environments. Specific bacterial taxa were enriched in the guts of mud crabs sampled from different salinity settings. Furthermore, functional enrichment analyses also showed that only gut bacteria had significant differences of functions in different salinity levels (high salinity versus low salinity), playing critical roles of signal transduction, and cell growth and death pathways. These results suggested that the microbiota in guts of crabs are more prone to variations in the salinity. These findings provide a comprehensive understanding of the composition and plasticity of mud crabs microbiota, which could lead to innovative strategies development for boosting the aquaculture industry of mud crabs.

Published in Ecological Indicators

ISSN: 1470-160X (Print); 1872-7034 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Ecology
Website: https://www.sciencedirect.com/journal/ecological-indicators

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