Department of Genetics, Stanford University, Stanford, United States
Karl Deisseroth
Department of Bioengineering, Stanford University, Stanford, United States; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, United States; Howard Hughes Medical Institute, Stanford University, Stanford, United States
Department of Genetics, Stanford University, Stanford, United States; Glenn Laboratories for the Biology of Aging at Stanford, Stanford, United States; Wu Tsai Neurosciences Institute, Stanford University, Stanford, United States
The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here, we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci in order to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.
