Importance of soil amendments with biochar and/or Arbuscular Mycorrhizal fungi to mitigate aluminum toxicity in tamarind (Tamarindus indica L.) on an acidic soil: A greenhouse study

Abstract

Tamarindus indica L. is a forest plant species widely used in semi-arid regions and has an important socio-economic role. A 90 d greenhouse pot experiment was conducted to evaluate the efficiency of soil amendments with biochar and/or three Arbuscular Mycorrhizal Fungi (AMF) strains; Rhizophagus fasciculatus (Rf), Rhizophagus aggregatus (Ra), and Rhizophagus irregularis (Ri) on T. indica grown under aluminum stress. The amendments consisted of 5% biochar and 20 g kg−1 AMF as (i) control; (ii) biochar; (iii) biochar + Rf; (iv) biochar + Ra; (v) biochar + Ri; (vi) Rf; (vii) Ra; (viii) Ri. The treatments with biochar significantly (P < 0.05) increased soil pH and reduced the content of soil exchangeable Al3+ relative to the control and exclusive AMF treatments. All the treatments improved total nitrogen and phosphorus uptake by roots and shoot of T. indica and resulted in improved plant growth and root/shoot dry weight. The ability of biochar to enhance the soil's water-holding capacity played a key role in improving the intensity of mycorrhization. Overall, biochar amendments significantly improved the photosynthetic potential of T. indica and the activities of antioxidant enzymes compared to other treatments. Thus, the combined effects of enhanced (a) soil physicochemical parameters, (b) mycorrhization, (c) nutrient uptake, (d) photosynthetic potential, and (e) antioxidant activities played an important role in mitigating Al-related stress to improve the growth of T. indica. Therefore, the application of biochar in combination with AMFs can serve as a strategy for ensuring plant biodiversity in acid and Al-toxic soils in arid and semi-arid regions in Africa.

Keywords

• Tamarindus indica
• Biochar
• Rhizophagus fasciculatus
• Rhizophagus aggregatus
• Rhizophagus irregularis
• Mycorrhization