Fabrication of Polysaccharide-Based Coaxial Fibers Using Wet Spinning Processes and Their Protein Loading Properties

Abstract

Fibers composed of polysaccharides are a promising candidate to be applied for biomaterials such as absorbable surgical sutures, textile fabrics, and hierarchical three-dimensional scaffolds. In this work, in order to fabricate biocompatible fibers with controlled-release abilities, the fabrication of coaxial fibers of calcium alginate (ALG-Ca) and polyion complexes (PICs) consisting of chitosan (CHI) and chondroitin sulfate C (CS), denoted as ALG-PIC fibers, by using a wet spinning process, and the evaluation of their molecular loading and release behavior were performed. The diameter and mechanical strength of the obtained ALG-PIC fibers increased with increasing concentrations of the CHI solution for PIC coatings. This indicated that higher concentrations of the CHI solution afforded a thicker PIC coating layer. Further, fluorescein isothiocyanate labeled-bovine serum albumin (FITC-BSA)-loaded ALG-PIC fibers were successfully prepared. The release behavior of FITC-BSA in the fibers exhibited a slower rate at the initial state than that in ALG-Ca, indicating that PIC coatings suppressed an initial burst release of the loading molecules. Accordingly, the fabricated coaxial fibers can be utilized as sustained-release drug carriers.

Keywords

• coaxial fiber
• polysaccharide
• polyion complex
• wet spinning process
• drug carrier