Recent advances in electrochemiluminescence imaging analysis
Chengda Meng,
Sara Knežević,
Fangxin Du,
Yiran Guan,
Frédéric Kanoufi,
Neso Sojic,
Guobao Xu
Affiliations
Chengda Meng
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China
Sara Knežević
University of Bordeaux, Bordeaux INP, CNRS, UMR 5255, Site ENSCBP, 33607 Pessac, France
Fangxin Du
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China
Yiran Guan
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China
Frédéric Kanoufi
Université Paris Cité, ITODYS, CNRS, F-75013 Paris, France
Neso Sojic
University of Bordeaux, Bordeaux INP, CNRS, UMR 5255, Site ENSCBP, 33607 Pessac, France; Corresponding authors.
Guobao Xu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China; Corresponding authors.
Electrochemiluminescence (ECL) has been widely applied in imaging owing to features that distinguish it from other microscopic techniques and electrochemical methods, including its high signal-to-noise ratio, remarkable sensitivity, wide linear range, high spatiotemporal resolution, and near-zero background light. Imaging technology based on ECL has been used in the fields of immunosensing, pathological cell detection, and drug analysis. Additionally, its simple operation and ability to detect dynamic processes and catalytic sites strengthen its potential for research on material surfaces and interfaces, in vivo biological analysis, and cell visualization. At the same time, the emergence of a variety of nanomaterials and new microscopic analysis equipment has further promoted the development of high-resolution ECL imaging technology. This paper introduces the development of ECL technology and the mechanisms of the main ECL systems. It then describes various forms of ECL imaging methods, and reviews research progress on ECL imaging technology in the fields of single-particle imaging, fingerprint structure analysis, and single-cell microscopic imaging. Finally, the authors offer their views about the prospects for ECL imaging technology.
