Screening thepresence of avirulence genes from blast fungal isolates, Magnaporthe oryzae, in Vietnam.

Abstract

The rice blast fungus, Magnaporthe oryzae, is the causal agent of severe damage in worldwide rice growing area. The resistance to blast infection has been determined by the relationship between the R resistant gene of rice and avirulence gene AVR of phytopathogenic fungi based on the “gene for gene” theory. The main objective of this study was to determine the presence/absence of AVR genes in the rice blast fungal isolates in Vietnam. The screening results of 30 fungal isolates collected from the south, the center and the north of Vie103-111tnam by PCR method indicated that the rates of presence/absence of the six avirulent genes varied clearly. In this study, AVR-Pik along with the CDS sequence of this gene (AVR-Pik CDS) was found to be nearly 99.67%, meanwhile the AVR-Pizt was only 36.67%. This result plays an important role in understanding the diversity of the AVR genes in M.oryzaethat could be helpful inthe selection and development of blast resistant rice varieties.

Keywords

• avr-pik cds
• avr-pik
• gen không độc
• m.oryzae
• nấm đạo ôn.