Molecular characterization and microbial resistance of different bacterial isolates in some dairy products

Abstract

Bacterial contamination of milk and dairy products is a common problem. In the last two years, the foodborne diseases caused by the intake of milk and dairy products have been mostly disturbed with Salmonella entertica, Listeria monocytogenes Escherichia coli 0157:H7 and Campylobacter jejune. The study aims to isolate multidrug resistance (MDR) bacteria in dairy products and study of the molecular characterization of that isolates. MDR bacteria were found in 30 out of the 131 bacterial isolates. The incidence of MDR bacterial isolates revealed the abundance of Staphylococcus sp. with 43.3%, Bacillus sp 16.7%, Salmonella 13.3%, E. coli 10 %, Enterococcus 6.7 % Psedoumonas 3.3 %, Shegella 3.3 % and Proteous 3.3 %. Molecular studies of genes presence or absence for class A contain TEM, CTX and BSHV, class B contain VIM, IMP, KPC and NDM, class C contain FOX and class D contain OXA-10, OXA-24 and OXA-58 were tested. NDM, TEM, CITM and OXA -10 genes were the most abundant the selected bacterial isolates. The results of this study indicate that cheese made from unpasteurized milk can pose a significant risk to consumers. Product manufacturing processes should be subject to health control-to-control pathogens. The novelty in this work depend on screening of gene responsible of the resistance from the bacteria isolated from dairy product using the molecular technique.

Keywords

• multidrug resistance
• gene
• bacteria
• milk
• dairy products