Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo

Boxian Huang; Chunyan Jiang; Aiqin Chen; Yugui Cui; Jiazi Xie; Jiandong Shen; Juan Chen; Lingbo Cai; Tingting Liao; Song Ning; Shi-Wen Jiang; Guoping Fan; Lianju Qin; Jiayin Liu

doi:10.1016/j.tjog.2015.08.003

Taiwanese Journal of Obstetrics & Gynecology (Oct 2015)

Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo

Boxian Huang,
Chunyan Jiang,
Aiqin Chen,
Yugui Cui,
Jiazi Xie,
Jiandong Shen,
Juan Chen,
Lingbo Cai,
Tingting Liao,
Song Ning,
Shi-Wen Jiang,
Guoping Fan,
Lianju Qin,
Jiayin Liu

Affiliations

Boxian Huang: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Chunyan Jiang: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Aiqin Chen: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Yugui Cui: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Jiazi Xie: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Jiandong Shen: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Juan Chen: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Lingbo Cai: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Tingting Liao: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Song Ning: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Shi-Wen Jiang: Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA
Guoping Fan: Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
Lianju Qin: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
Jiayin Liu: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China

DOI: https://doi.org/10.1016/j.tjog.2015.08.003
Journal volume & issue: Vol. 54, no. 5
pp. 505 – 511

Abstract

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Objective: Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders. Materials and methods: In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of mosaic trisomy 9 embryos. Results: A karyotype analysis showed that the hESC line possessed a euploid (46 chromosomes). The undifferentiated hESCs exhibited long-term proliferation capacity and expressed typical markers of OCT4, TRA-1-60, and TRA-1-81. In vitro embryoid-body (EB) formation, differentiation, and in vivo teratoma production confirmed the pluripotency of the hESC line. The data represented here are the first detailed report on the characterization and differentiation of one Chinese hESC line generated from mosaic trisomy 9 embryos. Conclusion: Our study showed that chromosomally aberrant embryos could generate a normal hESC line, which would be useful in investigating gene function and embryo development.

Published in Taiwanese Journal of Obstetrics & Gynecology

ISSN: 1028-4559 (Print)
Publisher: Elsevier
Country of publisher: Taiwan, Province of China
LCC subjects: Medicine: Gynecology and obstetrics
Website: http://www.journals.elsevier.com/taiwanese-journal-of-obstetrics-and-gynecology/

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