Molecules (Dec 2022)

MALDI-TOF Mass Spectrometry for the Diagnosis of Citrus Canker Caused by <i>Xanthomonas citri</i> subsp. <i>citri</i>

  • Edenilson dos Santos Niculau,
  • Douglas Ferreira,
  • Edson Rodrigues-Filho,
  • Franklin Behlau,
  • Rodrigo Facchini Magnani,
  • Leonardo Toffano,
  • Evandro Luis Prieto,
  • João Batista Fernandes,
  • Maria Fátima das Graças Fernandes da Silva

DOI
https://doi.org/10.3390/molecules27248947
Journal volume & issue
Vol. 27, no. 24
p. 8947

Abstract

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Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a disease that causes serious problems to the global citrus industry. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS) has been used in human medicine to diagnose various diseases caused by both fungi and bacteria. In agriculture, this technique has potential for the diagnosis of diseases due to the low cost of large-scale analysis and quickness. This study showed that MALDI-TOF MS combined with chemometric analysis was effective for differentiating the macromolecule profile of orange leaves with canker lesions, healthy leaves, and leaves with phytotoxicity symptoms, proving that this technique may be used for the rapid diagnosis of citrus canker.

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