Development of an HPV Genotype Detection Platform Based on Aggregation-Induced Emission (AIE) and Flow-Through Hybridization Technologies
Chun-Ho (Charlie) Ma,
Liejun Li,
Shuheng Cai,
Pei Lin,
Wing-Ki (Kristy) Lam,
Tsz-Him (Ronald) Lee,
Tsz-Kin (Ryan) Kwok,
Longxu Xie,
Tit-Sang (Tom) Kun,
Ben-Zhong Tang
Affiliations
Chun-Ho (Charlie) Ma
Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Liejun Li
Guangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China
Shuheng Cai
Guangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China
Pei Lin
Guangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China
Wing-Ki (Kristy) Lam
Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Tsz-Him (Ronald) Lee
Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Tsz-Kin (Ryan) Kwok
Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Longxu Xie
Guangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China
Tit-Sang (Tom) Kun
Guangzhou Hybribio Biotech Limited, No 71, Fenghuang 3rd Road, Sino-Singapore Guangzhou Knowledge City, Guangzhou 510000, China
Ben-Zhong Tang
Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
Genetic mutations can cause life-threatening diseases such as cancers and sickle cell anemia. Gene detection is thus of importance for disease-risk prediction or early diagnosis and treatment. Apart from genetic defects, gene detection techniques can also be applied to gene-related diseases with high risk to human health such as human papillomavirus (HPV) infection. HPV infection has been strongly linked to cervical cancer. To achieve a high-throughput HPV gene detection platform, the flow-through hybridization system appears to be one of the commercialized diagnostic techniques for this purpose. The flow-through hybridization technique is based on a vacuum-guided flow of DNA fragments which is continuously directed toward the oligoprobes that are immobilized on the testing membrane. However, the conventional colorimetric method and signal read-out approach suffers a problem of low sensitivity. On the contrary, fluorescence approaches allow more sensitive detection and broad sensing ranges. In this work, a fluorescent dye HCAP, which possesses aggregation-induced emission (AIE) properties and is responsive to alkaline phosphatase, was developed and applied to the flow-through hybridization platform to achieve HPV genome diagnosis of clinical samples. Also, an automatic membrane reader was constructed based on the AIE-based diagnosis platform which can identify the diagnostic result of patient DNA with a total concordance rate of 100% in the clinical trial.
