Neuropsychiatric Disease and Treatment (May 2020)

Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.

  • Zhang Y,
  • Liu L,
  • Liang C,
  • Zhou L,
  • Tan L,
  • Zong Y,
  • Wu L,
  • Liu T

Journal volume & issue
Vol. Volume 16
pp. 1239 – 1248

Abstract

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Yongcang Zhang,1,2,* Lan Liu,2,3,* Cuiting Liang,2 Lingyu Zhou,2 Lixia Tan,2 Yonghua Zong,4 Lili Wu,5 Tonghua Liu4,5 1Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, People’s Republic of China; 2Medical College, Tibet University, Lhasa 850000, People’s Republic of China; 3West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People’s Republic of China; 4Tibet Traditional Medicine University, Lhasa 850000, People’s Republic of China; 5Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lili WuKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of ChinaEmail [email protected] LiuTibet Traditional Medicine University, Lhasa 850000, People’s Republic of ChinaKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China Email [email protected]: The unique geographical environment at high altitudes may cause a series of diseases, such as acute altitude reaction, cerebral edema, and pulmonary edema. Gymnadenia conopsea (L.) R. Br. has been reported to have an effect on high-altitude hypoxia. However, the molecular mechanism, especially the expression of long noncoding RNAs (lncRNAs), is not yet clear.Methods: The expression profiles of lncRNAs in high-altitude hypoxia-induced brain injury mice treated with Gymnadenia conopsea (L.) R. Br. by using a microarray method.Results: A total of 226 differentially expressed lncRNAs, 126 significantly dysregulated mRNAs and 23 differentially expressed circRNAs were detected (> 2.0-fold, p< 0.05). The expression of selected lncRNAs, mRNAs and circRNAs was validated by qRT-PCR. KEGG analysis showed that the mRNAs coexpressed with lncRNAs were involved in inflammation and hypoxia pathways, including the HIF-1, PI3K-Akt, and NF-kappa B signaling pathways. The lncRNA-TF network analysis results indicated that the lncRNAs were regulated mostly by HMGA2, SRY, GATA4, SOX5, and ZBTB16.Conclusion: This study is the first to report the expression profiles of lncRNAs, mRNAs and circRNAs in mice with high-altitude hypoxia-induced brain injury treated with Gymnadenia conopsea (L.) R. Br. and may improve the understanding of the molecular mechanism of Gymnadenia conopsea (L.) R. Br. in treating high altitude hypoxia-induced brain injury.Keywords: expression profile, noncoding RNAs, high-altitude hypoxia, Gymnadenia conopsea (L.) R. Br

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