Construction of the PCR Test-System for the Detection of <i>Vibrio cholerae</i> O1 Toxigenic Strains, for Indication of Their Biovar and for Differentiation between Typical and Altered El Tor-Vibrio Strains

Abstract

Worked out is the multilocus PCR test-system which makes it possible to identify Vibrio cholerae O1 strains on the basis of rfb gene detection, to determine their biovar - either Classical or El Tor by testing cas3 or rtxC genes respectively, and at the same time to differentiate them into typical (which carry ctxBEltor gene) and genetically altered (which carry ctxBClass gene) variants. Efficacy and specificity of the test-system is demonstrated by the analysis of 64 natural V. cholerae strains of various serogroups and biovars, and 13 strains of enterobacteria and other species of Vibrio genus. Relation of the studied V. cholerae El Tor isolates to the typical or altered vibrio variants is proved out by ctxB gene sequencing.

Keywords

• <i>vibrio cholerae</i>
• multilocus pcr test-system
• identification and differentiation
• genetically altered vibrio variants