Heliyon (Oct 2018)
Effect of pennyroyal, savory and thyme essential oils on Japanese quail physiology
Abstract
An experiment was conducted to show the effects of different levels of pennyroyal, thyme and savory essential oils dietary supplementation on performance, organs weight, intestinal morphology, and serum lipids in quails. A total of 550 day-old Japanese quail chicks were allocated into 11 dietary treatments of 5 replications (10 birds in each cage) under a randomized experimental design. The treatments were a basal diet (Control), or 3 levels (200, 300, and 400 ppm) of thyme essential oil (TO), or 3 levels (200, 300, and 400 ppm) of savory essential oil (SO), or 3 levels (200, 300, and 400 ppm) of pennyroyal essential oil (PO) added separately to the basal diet, or also the basal diet supplemented with 100 ppm of flavophospholipol. Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were measured weekly and calculated totally for 35 d. The organs weight and morphology of intestine parts of one bird from each cage were measured on 35 d. At the same day, blood samples were collected and cholesterol and triglyceride values were separately determined for male and female quails. Although the addition of different levels of essential oils in quail's diet did not affect BWG, a significant decline in FI was observed in the group supplemented with 400 ppm of TO. At the same time, FCR significantly improved (P < 0.05) in the same group as well as in the group supplemented with the antibiotic. However, the organs weight was not significantly affected by the experimental treatments. Nevertheless, villi height of duodenum, jejunum and ileum was significantly increased and crypt depth was significantly decreased in the quails fed diets supplemented with different levels of TO and SO. The values of serum triglycerides decreased in both sexes in the groups that received diets supplemented with different levels of essential oils whereas the values of cholesterol decreased only in males of the essential oils supplemented groups. From the present observations it can be concluded that thyme and savory essential oils can improve FCR by decreasing FI through boosting the absorption of nutrients in intestine. These plant essential oils can replace antibiotic growth parameters without having any adverse effect on quail's health with thyme essential oil exerting the most effective activity.
