Cloning and functional verification of PdMYB57 from Paeonia delavay

Abstract

Abstract [Objective] As a peony breeding material, Paeonia delavayi has abundant flower color resources. Studying the regulatory effects of its MYB transcription factors on flower color will benefit molecular breeding of peony flower color. [Methods] Using the flowers of yellow and red P . delavayi as materials for hand sectioning. Based on the transcriptomic data of P . delavayi, a MYB transcription factor coding gene PdMYB57 was obtained through sequence alignment. Function of this gene was verified through gene cloning, phylogenetic tree construction, qRT-PCR, transient expression, and HPLC. [Results] PdMYB57 gene has an ORF of 798 bp, encoding an unstable hydrophilic protein of 265 amino acids. PdMYB57 was clustered with the Arabidopsis SG6 subfamily, as well as MYB transcription factors in P . qiui PqMYB113, P . suffruticosa PsMYB57/PsMYB58, and Vitis vinifera VvMYBA1/VvMYBA2. PdMYB57 had a R2R3 conserved domain [R/K] Px [P/A/R] xx [F/Y] motif. PdMYB57 gene was highly expressed in sepals of red P . delavayi and leaves of yellow P . delavayi. HPLC analysis showed that tobacco leaves transiently expressed PdMYB57 gene contained cyanidin-3-O-rutinoside (Cy3R). [Conclusion] PdMYB57 gene encodes an R2R3-MYB transcription factor. PdMYB57 gene expression is tissue-specific and promotes anthocyanin synthesis in plants.

Keywords

• peaonia delavayi
• pdmyb57
• gene cloning
• transient expression
• hplc