Antioxidant, Antimicrobial, and Insecticidal Properties of Chemically Characterized Essential Oils Extracted from <i>Mentha longifolia</i>: In Vitro and In Silico Analysis
Meryem Tourabi,
Ghizlane Nouioura,
Hanane Touijer,
Asmae Baghouz,
Asmae El Ghouizi,
Mohamed Chebaibi,
Meryem Bakour,
Driss Ousaaid,
Khalid S. Almaary,
Hiba-Allah Nafidi,
Mohammed Bourhia,
Khallouki Farid,
Badiaa Lyoussi,
Elhoussine Derwich
Affiliations
Meryem Tourabi
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Ghizlane Nouioura
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Hanane Touijer
Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Asmae Baghouz
Laboratory of Biotechnology, Conservation, and Valorization of Natural Resources, Department of Biology, Faculty of Science Dhar El Mahraz, University of Sidi Mohamed Ben Abdellah, B.P. 1796 Atlas, Fez 30003, Morocco
Asmae El Ghouizi
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Mohamed Chebaibi
Ministry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez 30000, Morocco
Meryem Bakour
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Driss Ousaaid
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Khalid S. Almaary
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Hiba-Allah Nafidi
Department of Food Science, Faculty of Agricultural and Food Sciences, Laval University, Quebec City, QC G1V 0A6, Canada
Mohammed Bourhia
Department of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune 70000, Morocco
Khallouki Farid
Ethnopharmacology and Pharmacognosy Team, Department of Biology, Moulay Ismail University of Meknes, Errachidia 52000, Morocco
Badiaa Lyoussi
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
Elhoussine Derwich
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30003, Morocco
The present study aimed to explore the phytochemical profile, and evaluate the antioxidant, antimicrobial, and insecticidal properties, of Moroccan Mentha longifolia L. essential oil (ML-EO) using in vitro and in silico assays. Noteworthily, as chromatography (GC-MS/MS) revealed that ML-EO is majorly composed of piperitenone oxide (53.43%), caryophyllene (20.02%), and (−) germacrene D (16.53%). It possesses excellent antioxidant activity with an IC50 of 1.49 ± 0.00 for DPPH and 0.051 ± 0.06 μg/mL for ABTS. Moreover, the RP and TAC activities were 0.80 ± 0.01 μg/mL and 315.532 ± 0.00 mg EAA/g, respectively. ML-EO exhibited a potent antimicrobial effect, specifically against Pseudomonas aeruginosa. It also exhibited strong antifungal ability, especially against Candida albicans. Regarding insecticidal activity, for ML-EO, a dose of 20 µL/mL produced a complete reduction in fecundity, fertility, and emergence of adult C. maculatus with mortality rates reaching 100%. In silico results showed that the antioxidant activity is mostly attributed to α-Cadinol, the antibacterial efficiency is attributed to piperitenone oxide, and antifungal capacity is related to cis-Muurola-4(15),5-diene and piperitenone oxide. Accordingly, ML-EO has high potential to be used as an alternative for preserving food and stored grain and protecting them against microbes and insect pests in the food and pharmaceutical sectors.
